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imaging conditions widefield fluorescence microscopy  (Nikon)


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    Nikon imaging conditions widefield fluorescence microscopy
    Imaging Conditions Widefield Fluorescence Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 10098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Characterization of sEVs isolated from plasma of women with preeclampsia or eclampsia. sEVs were isolated from plasma of women with eclampsia (Eclamp, n=5), preeclampsia with (PE-Compl, n=5) or without (PE, n=5) organ complications, and normotensive controls (NP, n=5) using the ultracentrifugation and filtration method (see Methods). (A) Chart showing the size and concentration distribution of sEVs from the experimental groups. The inserted graph indicates particle concentration. Images are representative sEVs observed in transmission electron microscopy (TEM) from randomly chosen normotensive pregnancy (Ai) or preeclampsia (Aii) groups. Line in the images, 50 nm. (B) Representative images of sEVs marker (HSP70, CD81, Alix, TSG101, CD63). (C) Representative image of placental marker (PLAP). The loading control was Ponceau Red staining. (D) sEVs (100 μg/ml) were treated with a fluorescent dye (PKH67) as indicated in Methods and used to estimate the percentage of cells that uptake sEVs (i.e., PKH67 positive) in each visual camp. (E) Representative sEVs incorporation using ExoGlow-RNA selective marker at 37 °C (active incorporation) and 4 °C (background). In the graph ***P<0.0005 and ****P<0.0001. Every dot represents an individual subject. Values are reported as median ± interquartile range.

    Journal: Frontiers in Physiology

    Article Title: In vitro evidence that plasma of women with eclampsia disrupts the blood-brain barrier

    doi: 10.3389/fphys.2026.1778955

    Figure Lengend Snippet: Characterization of sEVs isolated from plasma of women with preeclampsia or eclampsia. sEVs were isolated from plasma of women with eclampsia (Eclamp, n=5), preeclampsia with (PE-Compl, n=5) or without (PE, n=5) organ complications, and normotensive controls (NP, n=5) using the ultracentrifugation and filtration method (see Methods). (A) Chart showing the size and concentration distribution of sEVs from the experimental groups. The inserted graph indicates particle concentration. Images are representative sEVs observed in transmission electron microscopy (TEM) from randomly chosen normotensive pregnancy (Ai) or preeclampsia (Aii) groups. Line in the images, 50 nm. (B) Representative images of sEVs marker (HSP70, CD81, Alix, TSG101, CD63). (C) Representative image of placental marker (PLAP). The loading control was Ponceau Red staining. (D) sEVs (100 μg/ml) were treated with a fluorescent dye (PKH67) as indicated in Methods and used to estimate the percentage of cells that uptake sEVs (i.e., PKH67 positive) in each visual camp. (E) Representative sEVs incorporation using ExoGlow-RNA selective marker at 37 °C (active incorporation) and 4 °C (background). In the graph ***P<0.0005 and ****P<0.0001. Every dot represents an individual subject. Values are reported as median ± interquartile range.

    Article Snippet: After this, slides were kept at 4 °C until analysis by fluorescent microscopy (40X magnification) (Motic Scientific, San Antonio, TX, USA).

    Techniques: Isolation, Clinical Proteomics, Filtration, Concentration Assay, Transmission Assay, Electron Microscopy, Marker, Control, Staining